| ABSTRACT: This study aimed to establish the mass cultivation of Wolffia brasiliensis under laboratory conditions, determine the sensitivity of reference substances (potassium dichromate, sodium chloride, and potassium chloride), and estimate the acute toxicity (LC50;7d) for the herbicide glyphosate, diquat, paraquat, imazapyr, 2,4-D, saflufenacil, imazamox, penoxsulam, metsulfuron-methyl, and atrazine. Three media were tested for culture establishment: Hoagland’s medium, 5.0 g L-1 NPK fertilizer (20-5-20), a mixture of Oxisol + organic substrate of decaying aquatic plant (2:1; v v-1). Sensitivity tests with reference substances and acute toxicity for herbicides were carried out after establishing the cultivation. The species W. brasiliensis presented an... |
